Composite

Part:BBa_K277134:Design

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-21)


3L.3_23.A1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 515
    Illegal EcoRI site found at 5425
    Illegal EcoRI site found at 6946
    Illegal EcoRI site found at 7817
    Illegal XbaI site found at 4918
    Illegal PstI site found at 4177
    Illegal PstI site found at 5767
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 515
    Illegal EcoRI site found at 5425
    Illegal EcoRI site found at 6946
    Illegal EcoRI site found at 7817
    Illegal PstI site found at 4177
    Illegal PstI site found at 5767
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 515
    Illegal EcoRI site found at 5425
    Illegal EcoRI site found at 6946
    Illegal EcoRI site found at 7817
    Illegal BglII site found at 1547
    Illegal BglII site found at 6926
    Illegal BglII site found at 7583
    Illegal BamHI site found at 1964
    Illegal BamHI site found at 3712
    Illegal BamHI site found at 6479
    Illegal XhoI site found at 4076
    Illegal XhoI site found at 5693
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 515
    Illegal EcoRI site found at 5425
    Illegal EcoRI site found at 6946
    Illegal EcoRI site found at 7817
    Illegal XbaI site found at 4918
    Illegal PstI site found at 4177
    Illegal PstI site found at 5767
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 515
    Illegal EcoRI site found at 5425
    Illegal EcoRI site found at 6946
    Illegal EcoRI site found at 7817
    Illegal XbaI site found at 4918
    Illegal PstI site found at 4177
    Illegal PstI site found at 5767
    Illegal NgoMIV site found at 5804
    Illegal AgeI site found at 4428
    Illegal AgeI site found at 6788
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 550
    Illegal BsaI.rc site found at 924
    Illegal BsaI.rc site found at 971


Design Notes

Constructed using USER assembly method described in RFC38


Source

Synthetic Yeast

References